http://10.7.186.7:8080/jspui/handle/123456789/19 Natural Science (Mathematics, astronomy, physics, chemistry, geology, paleontology, biology, zoology, and botany) 2026-04-04T19:46:10Z http://10.7.186.7:8080/jspui/handle/123456789/76 Title: Answers to a selection of problems from Classical Electrodynamics Authors: Van Wijk, Kasper Description: RLS Digital Repository http://edepository.rls.gov.rw/jspui 1996-01-01T00:00:00Z http://10.7.186.7:8080/jspui/handle/123456789/37 Title: Biology Authors: Johnson, Raven Description: PDF 2000-01-01T00:00:00Z http://10.7.186.7:8080/jspui/handle/123456789/36 Title: Basic Cell Culture Protocols Authors: Helgason, Cheryl D. (Editor); Miller, Cindy L. (editor) Abstract: There are four basic requirements for successful cell culture. Each of these will be briefly reviewed in this introduction. However, a more detailed description is beyond the scope of this chapter. Instead, the reader is referred to one of a number of valuable resources that provide the information necessary to establish a tissue culture laboratory, as well as describe the basic principles of sterile technique (1–4). The first necessity is a well-established and properly equipped cell culture facility. The level of biocontainment required (Levels 1–4) is dependent on the type of cells cultured and the risk that these cells might contain, and transmit, infectious agents. For example, culture of primate cells, transformed human cell lines, mycoplasma-contaminated cell lines, and nontested human cells require a minimum of a Level 2 containment facility. All facilities should Description: Cell culture is an invaluable tool for investigators in numerous fields. It facilitates analysis of biological properties and processes that are not readily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. The purpose of this chapter is to explain the basic principles of cell culture using the maintenance of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples. Procedures for thawing, culture, determination of cell numbers and viability, and cryopreservation are described. 2015-01-01T00:00:00Z http://10.7.186.7:8080/jspui/handle/123456789/28 Title: Rock Physics and phase relations: a handbook of physical constants Authors: Ahrens, Thomas J. Description: PDF - Curated at http://edepository.rls.gov.rw/jspui 1995-01-01T00:00:00Z